马立克病病毒对鸡NRAMP1和TNFSF13B基因转录水平的影响

doi:10.11843/j.issn.0366-6964.2014.10.014

Abstract

Abstract:

This experiment was designed to study the transcription of NRAMP1 and TNFSF13B genes in chickens infected with Marek’s disease virus (MDV)，and reveal the disease resistance mechanism of different breeds.The excellent local breeds，Jining Bairi chicken，Wenshang Luhua chicken and Shouguang chicken in Shandong province were selected as the materials，at the same time，the SPF White Leghorns were selected as control.One hundred chicken embryos were randomly selected，hatched，brood.Artificially MDV inoculation was conducted at the age of 7 days.Then the liver and spleen of the survivors were picked up after raised for 90 days for quantitative PCR detection.At the spleen tissues，the mRNA transcription of NRAMP1 gene of Wenshang Luhua chicken was significantly higher than that of Shouguang chicken and SPF chicken (P<0.05)；at the liver tissues，mRNA transcription of TNFSF13B gene of Jining Bai’ri chicken was significantly higher than that of the other three breeds (P<0.05).These results indicated that NRAMP1，the resistance candidate gene，was significantly transcripted at 90 days post MDV challenge，and can be the important factor for enhancing resistance；TNFSF13B，as the tumor suppressor gene，was significantly transcripted in Jining Bai’ri chicken which was the most capability tumor incidence breed and showed the highest level of TNFSF13B in the survivors.

CLC Number:

S858.315.3

YANG Ling，ZHANG Jiao，LIU Ning，DONG Zhong-dian，WANG Hui. The Effect of Marek’s Disease Virus on Transcription Levels of NRAMP1 and TNFSF13B in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1679-1683.

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References

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The Effect of Marek’s Disease Virus on Transcription Levels of NRAMP1 and TNFSF13B in Chickens

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